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. 2017 Sep 11;19(9):316. doi: 10.1007/s11051-017-3993-5

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Two-photon fluorescence confocal microscope image (a) and two-exponential fit FLIM image (b) of HUVEC cells incubated with Au NP 1.3 × 10¹² NP ml⁻¹ for 24 h (static conditions), collected at 3 μm from the coverglass; 3D reconstruction of a stack of 11 FLIM images (c). In FLIM images, the red signal is the amplitude of short lifetime contribution (assigned to Au NP emission) and the blue signal is the amplitude of long fluorescence lifetime term (assigned to residual cell autofluorescence)