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. 2017 Sep 7;10:285. doi: 10.3389/fnmol.2017.00285

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Copyright © 2017 Gao, Zhang, Xu, Yang, Zhang, Cheng, Zhou, Huang, Wang, Chen, Sun and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FluoroGold (FG) labeling of surviving retinal ganglion cells (RGCs) in flat-mounted retinas in the NC, Qcn, COHT and COHT+Qcn groups after 0, 1, 2 and 4 weeks of microbead injection. All images were captured at same magnification. Scale bar, 100 μm (A). (B) Quantification of FG-labeled RGCs. The data are given as the mean ± SD; ^#p < 0.05, ^##p < 0.01 and ^###p < 0.001 and *p < 0.05, **p < 0.01. (C) Whole flat-mounted retina. The point on the white circle shows the central position selected for the RGC count.