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(A) Representative images of JC-1 staining of RGCs with CoCl2 and Qcn treatment for 12 h and 48 h. All images were captured at the same magnification. Scale bars, 100 μm. (B) Quantitative analysis of the ∆ψm by the ratio of the red and green fluorescence. The bars indicate the mean ± SD. *p < 0.05 and **p < 0.01 for the hypoxia+Qcn group compared with the hypoxia group. (C) Fluorescence density of J-aggregates (y-axis) against JC-1 monomers (x-axis) displayed in a dot plot.
