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Shotgun sequencing validates the predicted microbial metabolic trends in a small subset of fecal samples from the different three groups (the control group, the model group, and the SASP group). A small subset of six stool samples were subjected to shotgun metagenomic sequencing via Hiseq 4000 platform. Of the 14 microbial metabolic modules, 11 retained the same under- or over-abundance trend predicted from 16S gene sequencing, excluding the biosynthesis of amino acids.
