Table 1.
Body weight and T-cell number (CD3+ and CD4+) in spleen, blood, and mesenteric lymph nodes (lymph) after TCDD (30 μg/kg) or vehicle (sesame oil) treatment of mice by oral gavage once every 4 days for 28 days.
| Group | Mouse wt (mg) | %CD3+ lymph | %CD4+ lymph | %CD3+ spleen | %CD4+ spleen | %CD3+ blood | %CD4+ blood |
|---|---|---|---|---|---|---|---|
| UC Veh | 28.3 ± 0.8 | 48.2 ± 3.2 | 27.3 ± 1.4 | 27.8 ± 1.3 | 14.8 ± 0.9 | 18.0 ± 1.7 | 10.5 ± 0.6 |
| B Veh | 27.1 ± 0.6 | 45.2 ± 9.5 | 18.7 ± 4.6 | 29.2 ± 1.0 | 17.0 ± 0.6 | 22.9 ± 5.6 | 11.5 ± 2.9 |
| SFB+B Veh | 28.5 ± 0.6 | 48.8 ± 3.9 | 25.7 ± 2.2 | 25.0 ± 2.3 | 12.5 ± 1.4 | 20.7 ± 1.8 | 9.6 ± 1.0 |
| UC TCDD | 25.3 ± 0.6∗ | 43.1 ± 3.6 | 20.4 + 2.1 | 19.9 ± 2.5∗ | 10.3 ± 1.2∗ | 20.8 ± 2.3 | 9.8 ± 1.0 |
| B TCDD | 24.5 ± 1.0∗ | 72.9 ± 1.5∗ | 34.6 ± 1.1∗ | 23.9 ± 1.2∗ | 13.7 ± 1.0∗ | 20.7 ± 0.9 | 10.0 ± 1.0 |
| SFB+B TCDD | 26.1 ± 0.8∗ | 49.1 ± 4.0 | 25.6 ± 1.9 | 24.3 ± 2.3 | 12.8 ± 1.2 | 25.0 ± 2.0 | 12.0 ± 1.0 |
Numbers indicate mean and standard error. Abbreviations identify mice that remained uncolonized (UC) or were mono-colonized with B. fragilis (B) and co-colonized (SFB+B). Vehicle dosed mice (Veh) and TCDD dosed mice (TCDD). ∗Significant difference between TCDD and vehicle-dosed groups (p <0.05).