Table 1.

FISH oligonucleotide probes used in this study.

Probe	Target bacterial group/species	Target sequence (5′–3′)	Hybridization/washing T (°C)	Reference
Bifl64	Bifidobacterium spp. (BIF)	CATCCGGCATTACCACCC	50–50	Langendijk et al., 1995
Erec482	Clostridium clusters XIVa+b (ERE)	GCTTCTTAGTCARGTACCG	50–50	Franks et al., 1998
Labl58	Lactobacillus/Enterococcus spp. (LAB)	GGTATTAGCAYCTGTTTCCA	50–50	Harmsen et al., 1999
Chisl50	Clostridium histolyticum group clusters I, II (CHI)	TTATGCGGTATTAATCTYCCTTT	50–50	Franks et al., 1998
Bac303	Bacteroides–Prevotella group (BAC)	CCAATGTGGGGGACCTT	46–48	Manz et al., 1996
Eub338 I∗	Most bacteria (EUB)	GCTGCCTCCCGTAGGAGT	46–48	Daims et al., 1999
Eub338 II∗	Most bacteria (EUB)	GCAGCCACCCGTAGGTGT	46–48	Daims et al., 1999
Eub338 III∗	Most bacteria (EUB)	GCTGCCACCCGTAGGTGT	46–48	Daims et al., 1999

^∗These probes were used in equimolar concentrations (50 ng/mL). Formamide (35%) was included in the hybridization buffer.