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. 2017 Sep 7;7:401. doi: 10.3389/fcimb.2017.00401

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Copyright © 2017 Lee, Sng, Lee, Molton, Chan, Kalimuddin, Izharuddin, Lye, Archuleta and Gan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intracellular survival of different hvKP capsule types in healthy and poorly controlled type 2 DM PBMCs. PBMCs isolated from healthy donors (n = 8; mean age = 58.1 ± 8.6 years; normal fasting blood glucose and HbA1c levels) or type 2 DM donors with poor glycemic control (n = 10; mean age = 60.3 ± 9.5 years; mean HbA1c = 9.8 ± 3.2%) were infected with the K1 strain SGH04 and the K28 strain NUH29. Kanamycin was added at 2-h post-infection to kill off extracellular bacteria, and the number of intracellular bacteria was quantified at 4-, 8-, and 24-h post-infection. (A) Percentage killing between 4 and 8 h post-infection. (B) Percentage killing between 4 and 24 h post-infection. Error bars represent SEM. ^*p < 0.05, ^**p < 0.01.