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Map and restriction profile of the expression vector to overexpress the tau gene. (A) Physical map of the pVAX-Tau vector, which is based in the pVAX backbone and drives the expression of tau gene by the CMV promoter. (B) Restriction profiles of the constructed pVAX-Tau vector; the release of the 1.4 kb fragment upon EcoRI/ApaI restriction indicated the presence of the tau gene, which was confirmed by conventional sequencing.
