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. 2017 Sep 7;8:1733. doi: 10.3389/fmicb.2017.01733

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Copyright © 2017 Zhou and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of specific anti-T. gondii IgG1 and IgG2a in immunized mice. The sera of 20 mice of each group were collected from 2 weeks after the final immunization and the IgG subtypes levels were analyzed by ELISA. T. gondii-specific IgG1 and IgG2a were used in the detection. Results are expressed as means of the OD490 ± SD statistically significant differences (p < 0.05) compared to the control groups. Each sample was tested three times. ^∗Compared with PBS or pEGFP-C1, p < 0.05 and ^∗∗compared with pSAG4 or peptide, p < 0.05.