Table 2.

IC50 values for SAG4 and SAG1 binding to MHC class II molecules obtained using the Immune Epitope Database^a.

MHC II Alleleb	Start-Stopc		Percentile Rankd
	SAG1	SAG4	SAG1	SAG4
H2-IAb	26–40	61–75e	2.15	0.31
H2-IAd	21–35	42782	0.34	0.66
H2-IEd	14–28	15–29	18.45	3.61
HLA-DRB 1∗01:01	43095	43064	0.88	0.25

^aThe Immune Epitope Database (http://tools.immuneepitope.org/mhcii). The prediction was run for three times. ^bH2-IAb, H2-IAd, and H2-IEd alleles are mouse MHC class II molecules; the HLA-DRB1^∗01:01 allele is a human MHC class II molecule. ^cWe chose 15 amino acids for analysis each time. ^dLow percentile = high level binding, high percentile = low level binding. ^eThe sequence of the peptide (SRPLEYIPPNPSQVL) used in the study.