Figure 1.

Ontogenic and regenerating zebrafish fins express Tmem184a in the vasculature. Representative confocal microscopy images of cryogenic transverse sections of regenerating and ontogenic Tg(Fli1:EGFP) fins. All sections were stained with a GFP antibody to enhance EC fluorescence/visualization along with DAPI to easily identify fin rays. (A) Sections were stained for Tmem184a using a commercial antibody. (B) Sections were stained for Tmem184a using a zebrafish-specific antibody (ZF-CTD). Each image shows one individual fin ray. The arrowhead points to a mature artery, and the arrows mark mature veins. Scale bars = 50 μm. DAPI staining is blue, EC staining is green, and Tmem184a staining is red for all images. Sections from more than 10 individual fish were immunostained and analyzed for each combination of antibodies.