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Two MOs against Tmem184a sufficiently knock down protein expression. (A) Validation of Tmem184a knockdown by western blotting of ontogenic and 4 dpa fin lysates with the ZF-CTD antibody. (B) Quantitation of Tmem184a band intensity/actin band intensity. Data are representative of more than three independent experiments with 8–12 fish injected per experiment. Graphs are shown as average ratio ± S.E.M. **p < 0.01 compared to Con MO.
