Table 2.
Genes that were over-expressed or deleted from the chromosome.
| Gene name | Present in strain | Locus tag protein ID | Protein size (aa/kDa) | Growth phase¥ | Gene presence (Pr)¥ Gene absence (Ab) | Predicted phenotype¥,$ | Detected phenotype*,$ | Detected in growth phase* |
|---|---|---|---|---|---|---|---|---|
| OVEREXPRESSION IN L. lactis NZ9000 | ||||||||
| Cell surface protein precursor | B40 | B40_0084 LITC01000011 KZK48299.1 | 930/102.17 | ST | Pr | ZP▾ | CSH▴ | ST |
| Ribose 5-phosphate isomerase A (rpiA) | KF147 | KF147_0667 ABX75739.2 | 243/26.95 | EX | Ab | ParaCN▴ | CSH▴ | ST |
| Internalin, putative (LPXTG motif) | KF282 | KF282_0409 - | 559/61.28 | ST | Pr | ZP▴ | CSH▴ | EX, ST |
| Hypothetical protein (yreB) | IL1403 | L128699 AAK05785.1 | 314/35.64 | EX | Pr | CSH▴ | None | – |
| Cell wall surface anchor family protein | KF147 | LLKF_0311 ADA64081 | 809/87.46 | EX EX | Pr Ab | CSH▴ E24▴ | CSH▴ ParaCN▾ NaCN▾ NaCN90C▾ | EX, ST ST ST ST |
| Endo-beta-N-acetylglucosaminidase (ypcCD) | KF147 | LLKF_1605 ADA65249 | 923/102.53 | ST ST | Ab Ab | ParaCN▴ NaCN▴ | CSH▴ | ST |
| KNOCKOUT IN L. lactis MG1363 | ||||||||
| Conjugal transfer protein (traG) | MG1363 | llmg_1383 CAL97970 | 612 | EX | Ab | ParaCN▾ | ParaCN▾ NaCN▾ NaCN90C▾ | EX EX EX |
| Cell surface protein precursor | MG1363 | llmg_1096 CAL97690 | 387 | ST | Ab | ZP▾ | No | – |
| Hypothetical protein | MG1363 | llmg_1093 CAL97687.1 | 334 | ST | Pr | NaCN▴ | NaCN▾ | EX, ST |
| Sortase SrtA (srtA) | MG1363 | llmg_1449 | 250 | – | – | – | No | – |
These columns indicate if either the presence or absence of a particular gene of cells from exponential (EX) or stationary (ST) growth phase resulted in an altered phenotype.
These columns indicate phenotypic changes that were detected in a particular growth phase in engineered strains where the indicted genes were either overexpressed or deleted.
ZP, charge (mV); ParaCN, attachment to paracaseinate (%); NaCN, attachment to sodium caseinate (%); NaCN90C, attachment to sodium caseinate heated at 90°C for 10 min (%); CSH, cell surface hydrophobicity (%); E24, emulsion stability for 24 h (%); ▴, cell surface property increases; ▾, cell surface property decreases. All phenotype changes indicated are significant with p < 0.01.