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. 2017 Sep 7;8:1543. doi: 10.3389/fpls.2017.01543

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Copyright © 2017 Migdal, Skibior-Blaszczyk, Heidorn-Czarna, Kolodziejczak, Garbiec and Janska.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression level for selected genes encoding mitochondrial proteases in 4-week-old oct1-1, imp1a-1, icp55-1 and oma1-1 mutants grown under LD, 22°C, compared to wild-type plants. (A) The transcript level of mitochondrial ATP-independent and ATP-dependent proteases. Analysis was performed by quantitative real-time PCR. Relative abundance of transcripts is expressed as log2 ratios. Mean values ± SD from four independent experiments are shown. (B) Abundance of PREP (PREP1 and PREP2), OOP, RBL12, FTSH3, FTSH10 and FTSH4 proteases in mitochondria of WT and studied mutants. Ponceau S serves as a loading control. 30 μg of mitochondrial protein was loaded on gel. Protein amount was quantified densitometrically and values are given as percentage of the value obtained for wild-type plants (set as 100%). Mean values ± SD from three independent experiments are shown. ^∗p ≤ 0.05.