Figure 4.

The pAKT protein expression level changes influenced by lncRNA260-specific siRNA in the H/R cardiomyocytes injury. (A) The pAKT western blot result of the transfected cardiomyocytes with lncRNA260 siRNAs in the H/R procedure was displayed. The lncRNA260 siRNAs mediated pAKT protein increase. The β-actin was analyzed as loading control; (B) the effect of lncRNA260-specific siRNAs on the pAKT/β-actin protein ratio in H/R induced cardiomyocytes injury. The lncRNA260 siRNAs mediated pAKT/β-actin protein increase. Data are presented as the mean ± SD (n=5). *P<0.05, compared with H/R group and H/R + negative control transfection group; △P<0.05, compared with normal control group. H/R, hypoxic reoxygenation.