Figure 5.

The intracellular mechanisms underlying the protection effects of lncRNA260-specific siRNA on the H/R cardiomyocytes injury. (A) The Bcl-2 and Bax western blot results of the transfected cardiomyocytes with lncRNA260 siRNAs in the H/R procedure were displayed. The lncRNA260 siRNAs mediated Bcl-2 protein increase, and Bax protein decrease. The β-actin was analyzed as loading control; (B) the effect of lncRNA260 siRNAs on the Bcl-2/Bax protein ratio in H/R induced cardiomyocytes injury. The lncRNA260 siRNAs mediated Bcl-2/Bax protein increase. Data are presented as the mean ± SD (n=5). *P<0.05, compared with H/R group and H/R + negative control transfection group; △P<0.05, compared with normal control group. H/R, hypoxic reoxygenation.