Fig. 5.

Embelin/LY294002 combination inhibits growth of MDA-MB-231 Xenografts. Female nude mice at 6 weeks of age were injected S.C. with 10 million MDA-MB-231 cells. After one week, mice were divided into four groups; the first group only received DMSO vehicle alone; the second and third group were treated with either embelin (10 mg/kg) or LY294002 (10 mg/kg) and the fourth group were treated with combination of embelin and LY294002. (a) Volume of each tumour was measured every week. The average (n = 4) tumour volume of mice was calculated, * p < 0.05 inhibition of MDA-MB-231 tumour growth by combination of embelin and LY294002. (b) After 4 weeks of treatment, mice were sacrificed and tumour weights were measured,*p < 0.05 compared to vehicle-treated mice by Student’s t-test. (c) Representative tumour images of vehicle, embelin, LY294002 and combination of embelin and LY294002 treated mice. (d) Whole cell lysate from mice treated with different inhibitors were isolated and 10 μg protein were separated by SDS-PAGE, transferred to PVDF membrane, and immunoblotted with antibodies against XIAP, p-AKT, AKT, Bcl-Xl, Bcl-2, caspase-3 and Beta-actin