Detection of caspase 3, 7 in ATRT tumors cell lines following treatment with Vatalanib. ATRT cell lines ATRT-05 and ATRT-06 were exposed to Vatalanib (1 μM) for 48 hours (A–C) and 7 days (G–I). Caspase 3, 7 was detected in ATRT cell lines treated for 48 hours (A) or 7 days (G) using a Ready Probe to caspase 3, 7 (Molecular Probes). Dead cells were detected with NucRed® Dead 647 ReadyProbes® Reagent in ATRT cell lines treated with Vatalanib for 48 hours (B) or 7 days (H). No staining for caspase 3, 7 or dead cells were detected in cell lines not exposed to drug (D–F, J–L). 40x; Scale bar = 100 microns.