Extended Data Figure 4. Serial sectioning through most of a 7 dpf larval zebrafish brain.

a, Overview micrographs from a collection of 15,046 × ~50 nm-thick transverse serial sections that span 0.75 mm through a 7 dpf larval zebrafish. Surrounding part of the larval zebrafish (green dashed circle) with support tissue stabilized sectioning. Dashed lines indicate cropping. b, Volume rendering of aligned overview micrographs. Magenta and yellow planes correspond to reslice planes in c. Green plane corresponds to section outlined in a. c, Reslice planes through an aligned overview image volume reveal structures contained within the series and illustrate the sectioning plane relative to the horizontal (upper) and sagittal (lower) body planes. This series spans from posterior hindbrain through the anterior larval zebrafish, encompassing most of the brain. Dashed lines indicate where reslice planes intersect. d, Histograms depicting the number of lost, partial (missing any larval zebrafish tissue), or adjacent (lost-partial or partial-partial) events per bin of 50 sections throughout the series. In total, 6 (0.04%) sections were lost and 25 (0.17%) were partial for this series. No two adjacent sections were lost. Scale box: b, 250×250×250 µm³. Scale bars: a,c, 250 µm.