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. 2017 Sep 11;18:715. doi: 10.1186/s12864-017-4127-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Growth curves of parent strain and genome-reduced mutants. Growth trends of all 23 strains were divided into three groups. a Group 1: GP72(rpeA-) and MDS1–8. b Group 2: MDS9–18. c Group 3: MDS19–22. Strains were grown in KMB liquid medium adding 40 mg/L Km at 28 °C and 180 rpm. The values are presented as the means of three replicates, and the error bars indicate the standard deviation (SD)