Table 3.
Main bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Escherichia coli | ||
| DH5α | Cloning host; F− λ− endA1 glnX44(AS) hiE1 recA1 relA1 spoT1 gyrA96(NalR) rfbC1 deoR nupG Φ80(lacZΔM15) Δ(argF-lac)U169 hsdR17(r K − m K +) | TransGen Biotech, Beijing, China |
| S17–1 (λpir) | Donor strain; res − pro mod + integrated copy of RP4, mob+, used for incorporating constructs into P. chlororaphis | Lab stock; Hoffmann et al. |
| Pseudomonas chlororaphis | ||
| GP72 | P. chlororaphis GP72 wild-type strain | Lab stock; Liu et al. |
| GP72(rpeA-) | GmR, rpeA insertionally inactivation mutant of GP72 | Lab stock; Huang et al. |
| MDS5 | GmR, mutant of GP72(rpeA-) with deletion of 1.40% of the genome, including five secondary metabolic gene clusters | This study |
| MDS10 | GmR, mutant of GP72(rpeA-) with deletion of 3.52% of the genome | This study |
| MDS22 | GmR, mutant of GP72(rpeA-) with deletion of 10.29% of the genome | This study |
| Plasmid | ||
| pK18 mobsacB | Broad-host-range gene replacement vector, sacB, KmR |
Lab stock; Schafer et al. |
| pK18- MDXb | pK18 mobsacB containing MDX flanking region |
This study |
aAntibiotic markers: Km, kanamycin
bMDX means the corresponding multiple-deletion region