Fig. 2.
4E-BP loss of function enhances lifespan extension by cold. (A) Survival of 4e-bp null (4e-bp−/−) and control (4e-bp+/+) flies. Mean life extension by cold is enhanced in the null (Right). n = 554–601 flies. (B) Survival of a 4e-bp hypomorphic allele using RNAi and the ubiquitously expressed daughterless-GAL4/UAS-Dcr2 driver. Controls are hemizygous for each transgene. n = 156–363 flies. (C) Cox proportional hazards analysis of survival data. The negative coefficient for the interaction term indicates that the loss of function manipulations gain a greater benefit from cold than their respective controls. Genotype refers to the experimental cohort, 4e-bp−/− or 4e-bp RNAi/GAL4. Males were used in all studies. ***P < 0.001. Wald χ2 = 682.9, P < 10−6 (4e-bp−/−); χ2 = 263.5, P < 10−6 (RNAi).