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. 2017 Aug 21;114(36):9737–9742. doi: 10.1073/pnas.1618994114

Fig. 3.

Fig. 3.

Overexpression of activated 4E-BP diminishes lifespan extension by cold. (A and B) Survival at three temperatures of animals bearing the ubiquitous armadillo-GAL4 (A) or the ubiquitous, drug-inducible daughterless-GS GAL4 (B) driver expressing an active form of 4E-BP [d4E-BP(LL)]. GS is activated upon ingestion of the drug, RU486. Controls are hemizygous for each transgene (A) or fed vehicle instead of RU486 (B). Mean life extension by cold is inhibited with 4e-bp overexpression (Right). n = 100–208 flies (A); 48–98 flies (B). (C) Cox proportional hazards analysis of survival data. The positive coefficient for the interaction term indicates that 4E-BP activity attenuates the effect of cold. Genotype refers to the experimental cohorts. Males were used in all studies. **P < 0.01; ***P < 0.001. Wald χ2 = 135.1, P < 10−6 (A); χ2 = 92.4, P < 10−6 (B).