Fig. S3.

Characterization of pharmacological manipulations. (A) Rotenone treatment mimics the effect of cold by increasing the proportion of nonphosphorylated 4E-BP at 25 °C. n = 4–10 vials of flies per condition. (B) Dietary supplementation of rotenone or 4EGI-1 does not affect food intake. Feeding over 24 h was measured using radioisotope-labeling of the medium. n = 10 vials of flies per condition. (C) 4EGI-1 treatment mimics the effect of cold by increasing COX activity. n = 17–21 group of flies per condition. Canton-S males were used in all studies. *P < 0.05.