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. 2017 Aug 21;114(36):E7545–E7553. doi: 10.1073/pnas.1620898114

Fig. 4.

Fig. 4.

The plasma profile of CYP-dependent metabolites in CYP enzyme Tg mice. The plasma levels of 17,18-EEQ (A) and 19,20-EDP (B) in CYP-Tg mice [C57BL/6 background (abbreviated as BL6), Tie2-CYP2C8-Tg (abbreviated as 2C8), sEH null, and Tie2-sEH-Tg (abbreviated as sEHtg), respectively] were determined at 7 d after CNV induction by LC-MS/MS. Tie2-CYP2C8-Tg mice overexpress the monooxygenase CYP2C8 in endothelial cells, which promotes the metabolism of the primary LCPUFAs to their active downstream fatty acid metabolites (e.g., 17,18-EEQ and 19,20-EDP). sEH null mice lack expression of sEH, an enzyme that degrades these CYP-derived fatty acid metabolites into less bioactive vic-diols. Conversely, Tie2-sEH-Tg mice overexpress sEH. Mice were fed either a ω-3 LCPUFA-enriched diet, ω-6 LCPUFA-enriched diet, or control diet. Data are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. N.S., not significant. n = 3–4 mice per experimental group.