Fig. 7.

Administration of CYP-dependent ω-3 LCPUFA metabolites modulates leukocyte rolling velocity and adhesion molecule expression. (A and B) Cumulative frequency of leukocyte rolling velocity in a chamber coated with both P-selectin and ICAM-1 was assessed at day 3 after CNV induction. Mice were injected i.p. with (A) 17,18-EEQ (50 μg/kg/d), (B) 19,20-EDP (50 μg/kg/d), or PBS daily beginning immediately after CNV induction. Administration of both EEQ and EDP increased leukocyte rolling velocity compared with PBS groups (n = 3 mice per group). (C) Representative rendering depicting images of individual leukocytes tracked over 10 s. (D–G) Flow cytometric analysis of (D and E) CD11b and (F and G) CD18 expression on peripheral blood leukocytes 3 d after CNV induction in C57BL/6 mice injected i.p. with (D and F) 17,18-EEQ (50 μg/kg/d) or (E and G) 19,20-EDP (50 μg/kg/d). Administration of EEQ decreased CD18 expression and EDP decreased CD11b expression on leukocytes compared with PBS-injected groups. Data are presented as means ± SEM. n = 10 mice per group. MFI, mean fluorescence intensity values. *P < 0.05. (H and I) Real-time PCR analysis of (H) ICAM-1 and (I) E-selectin mRNAs in laser-captured CNV lesions at 7 d after CNV induction in C57BL/6 mice injected i.p. with 17,18-EEQ (50 μg/kg/d) and 19,20-EDP (50 μg/kg/d). Administration of 17,18-EEQ significantly decreased ICAM-1 and E-selectin expression compared with PBS-injected groups. Data are presented as means ± SEM. n = 6 mice per group. *P < 0.05.