Fig. S7.

Administration of CYP-dependent ω-6 epoxides modulates leukocyte rolling velocity and adhesion molecule expression. (A–C) Cumulative frequency of leukocyte rolling velocity in a chamber coated with both P-selectin and ICAM-1 were assessed at day 3 after CNV induction. Mice were injected i.p. with (A) 8.9-EET (50 μg/kg/d), (B) 11.12-EET (50 μg/kg/d), (C) 14.15-EET (50 μg/kg/d), or PBS daily beginning immediately after CNV induction. Administration of EETs decreased leukocyte rolling velocity compared with PBS groups (n = 3 mice per group). (D) Representative rendering depicting images of individual leukocytes tracked over 10 s. (E–J) Flow cytometric analysis of (E–G) CD11b and (H–J) CD18 expression on peripheral blood leukocytes 3 d after CNV induction in mice injected i.p. with (E and H) 8,9-EET (50 μg/kg/d), (F and I) 11,12-EET (50 μg/kg/d), or (G and J) 14,15-EET (50 μg/kg/d). Administration of EETs significantly increased CD18 expression on leukocytes compared with PBS-injected groups. Data are presented as means ± SEM. n = 10 mice per experimental group. MFI, mean fluorescence intensity values. *P < 0.05. (K and L) Real-time PCR analysis of (K) ICAM-1 and (L) E-selectin mRNAs in laser-captured CNV lesions at 7 d after CNV induction in injected i.p. with EETs (50 μg/kg/d for each group). Administration of EETs significantly increased ICAM-1 expressions and administration of 11,12-EET increased E-selectin expressions compared with PBS-injected group. Data are presented as means ± SEM. n = 6 mice per experimental group. **P < 0.01, ***P < 0.001. N.S., not significant.