Fig. 1.
TNF-α, IFN-γ, and IL-2 follow individual production kinetics. (A and B) Cytokine production of resting CD8+ OT-I T cells stimulated with 100 nM OVA peptide. Brefeldin A (BrfA) was present throughout the stimulation (A) or added for the last 0.5, 1, or 2 h of activation (B). (C) Cytokine profile analysis of T cells activated for 2, 4, or 6 h from B. (Upper) Percentage of T cells that produce at least one cytokine. (Lower) Cytokine production of responding T cells. Pooled data from three independently performed experiments (mean ± SD; n = 7 mice). (D) FACS-sorted CD44hi memory-like OT-I T cells activated with peptide-loaded bone marrow-derived dendritic cells. N4, SIINFEKL; T4, SIITFEKL (n = 5 mice). (E) Effector memory (EM, Left) and central memory (CM; Right) T cells from LCMV-infected mice (d60) were reactivated with GP33 and NP396 peptide (n = 5 mice).