Fig. 3.

PKC signaling promotes translation initiation. (A and B) 4E-BP1 expression in resting and OVA peptide-stimulated T cells (A), and in OVA-stimulated T cells pretreated for 30 min with rapamycin (B). (C and D) pmTOR and pS6 levels 30 min after T cell activation with OVA peptide (C), or with PMA/ionomycin (D). Indicated inhibitors were added 30 min before T cell activation. (E) 4E-BP1 expression of PMA/ionomycin activated T cells that were pretreated or not with indicated inhibitors. Representative of two independently performed experiments (A, B, and E) and 10 mice from three experiments (C and D).