NPC cells are susceptible to BRD4 inhibition. (A) C666-1 and NP69 cells were treated with indicated JQ1 with vehicle as control for 3 d. CellTiter-Glo luminescent was used to determine the cell viability. C666-1 cells treated with JQ1 grow significant slower than DMSO-treated cell (P < 0.01). JQ1 does not slow NP69 cell growth in the same time course. (B) Total RNAs from C666-1 and NP69 cells treated with JQ1 or vehicle were analyzed by qRT-PCR for ETV6, DNMT3A, EN2, and SGNB expression, normalized against GAPDH. DMSO-treated cells were set to 1. *P < 0.05, **P < 0.01.