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. 2017 Aug 21;114(36):E7499–E7505. doi: 10.1073/pnas.1707727114

Table S6.

Taxon-specific primer pairs used in this study with target marker, optimized annealing temperature, and amplified product size

Primer pairs Sequence, 5′–3′ Target group Locus Annealing temperature, °C Expected length, bp Source
LepF1 ATT CAA CCA ATC ATA AAG ATA TTG G Lepidoptera COI 49 658 Ref. 90
LepR1 TAA ACT TCT GGA TGT CCA AAA AAT CA
EF51.9 CAR GAC GTA TAC AAA ATC GG Gelechioidea EF1α 53 511 Ref. 91
EFrcM4 ACA GCV ACK GTY TGY CTC ATR TC Erebidae 55
Riodinidae 55.5
LepWg1 GAR TGY AAR TGY CAY GGY ATG TCT GG Gelechioidea Wg 60 403 Ref. 92
LepWg2 ACT ICG CAR CAC CAR TGG AAT GTR CA Erebidae 57
Riodinidae 54
LepWg1a_mod GAA TGT AAR TGT CAY GGY ATG TCY GG Erebidae Wg 53 403 This study
Lepwg2a_mod ACT GCG CAG CAC CAR TKG AAT GTG CA Riodinidae 54.3
LepWg1 GAR TGY AAR TGY CAY GGY ATG TCT GG Erebidae Wg 54 348 This study
Ere_Wg_mod GAT ACC CTC KIC CRC ARC Riodinidae 51.5