Table S1.

Comparative analysis of hair bundle geometric parameters in VHCs of Ush1g^+/− and Ush1g^−/− mice

	Ush1g+/−		Ush1g−/−
Geometric parameter	Mean ± SD	n	Mean ± SEM	n	Statistical comparison
Projected height, tallest row	5.8 ± 0.3	25	5.2 ± 0.2	58	0.12
Projected length, tallest row	9.6 ± 0.7	25	8.5 ± 0.4	58	0.19
Interstereocilia distance, tallest row	0.38 ± 0.04	25	0.33 ± 0.02	58	0.28
No. of stereocilia, tallest row	29.5 ± 2.7	25	32.2 ± 3.1	58	0.50
No. of rows	5.1 ± 0.2	24	4.7 ± 0.1	54	0.11
Distance between rows	0.79 ± 0.05	24	0.74 ± 0.04	54	0.48
Apical cell surface area	20.2 ± 3.2	25	24.6 ± 1.7	58	0.23

Measurements were based on scanning electron microscopy images of the utricle taken in Ush1g^−/− mice (n = 10) and Ush1g^+/− mice (n = 5) on P8. After selection of a representative set of hair bundles, various geometric parameters were extracted using a custom interface allowing a semiautomatized segmentation of stereocilia. Projected heights of the tallest stereocilia row were evaluated under similar angles of view for the two mutants. Statistical comparisons using the Student’s t test did not reveal any significant difference in the hair bundle parameters between the Ush1g^−/− and Ush1g^+/− mice. Values are given as mean ± SEM. All lengths and distances are given in micrometers, and areas are given in square micrometers; n is the number of cells analyzed.