Fig. S6.

Generation and characterization of pericyte-specific Cd146-KO mice. (A) Targeting strategy for generation of Cd146^{floxed/floxed} mice; shown are the WT locus of mouse Cd146 gene (Top) and the targeting construct (Bottom). A LoxP site (3′loxp) was cloned upstream of the promoter, and the frt-Neo-frt-loxp cassette was cloned downstream of exon 1. (B) Targeting strategy for generation of Pdgfrb^cre/+ mice. (C) Mating scheme to generate pericyte-specific Cd146-KO mice (Pdgfrb^cre/+Cd146^flox/flox, i.e., Cd146^PC-KO) and control WT littermates (Pdgfrb^+/+Cd146^flox/flox, i.e., Cd146^WT). (D) Genotyping of Cd146^PC-KO and Cd146^WT mice by PCR analysis of genomic DNA. A 595-bp fragment from WT Cre gene, a 550-bp fragment from WT Cd146 gene (WT Cd146), and a 502-bp fragment from floxed Cd146 gene (Mut Cd146) were PCR-amplified with specific primers. Genomic DNA from Pdgfrb^cre/+ mice was used as positive control (P.C.) for Cre analysis; genomic DNA from Cd146^{floxed/floxed} mice were used as positive control for Mut Cd146 analysis; genomic DNA from C57BL/6 mice were used as positive control for WT Cd146 analysis. H₂O was used as negative control (N.C.) for all three PCR analyses. Data represent three independent experiments.