Fig. 1.

Binding of LTA₄ in the active site of LTA₄H. (A and B) Different views of the active site of orthorhombic D375N (residues are in green) complexed with LTA₄ (orange) and acetate (ACT, violet). Yellow and red spheres represent Zn²⁺ and catalytic waters, respectively. mF_o-DF_c polder omit map contoured at 3.0 σ is shown in gray mesh around LTA₄ and ACT. (C and D) Different views of the active site of orthorhombic E271A-LTA₄ complex (residues are in cyan). (E) LTA₄ bound with its C1 carboxylate anchored to R563 in the active sites of D375N and (F) E271A. mF_o-DF_c omit map for Zn²⁺ and catalytic waters is shown in green mash at 3.0 σ. Distances are labeled in Å.