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. Author manuscript; available in PMC: 2017 Sep 12.
Published in final edited form as: Biochem J. 2017 Apr 19;474(9):1481–1493. doi: 10.1042/BCJ20160910

Figure 3. TMEM165 is targeted to lysosomal degradation after MnCl2 exposure.

Figure 3

(A) HeLa cells were treated for 8h with MnCl2 500 μM and/or chloroquine 100 μM, fixed and labeled with antibodies against TMEM165 (upper panels) and LAMP2 (middle panels) before confocal microscopy visualization. (B) Western blot analysis of the same experiment described in (A). Total cell lysates were prepared, subjected to SDS-PAGE and Western blot with the indicated antibodies. Right panel shows the quantification of TMEM165 protein level after normalization with actin (N = 2; *** = P value < 0,001).