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. Author manuscript; available in PMC: 2017 Sep 12.
Published in final edited form as: Biochem J. 2017 Apr 19;474(9):1481–1493. doi: 10.1042/BCJ20160910

Figure 4. TMEM165 lysosomal degradation is Rab7 and Rab5 independent.

Figure 4

HeLa cells were transfected with empy vector (mock), Rab7T22N-GFP or Rab5Q79L-GFP. 36H after transfection, cells were treated or not for 4h with MnCl2 500 μM. (A) Total cell lysates were prepared, subjected to SDS-PAGE and Western blot with the indicated antibodies. Right panel shows the quantification of TMEM165 protein levels after normalization with actin (N = 2; *** = P value < 0,001). (B) Immunofluorescence analysis by confocal microscopy of the same experiments as described in (A) (N = 2 ; n= 50).