(A) TMEM165 full length, short isoform and long isoform protein organization. Grey boxes represent transmembrane domains and the red boxes are the well conserved acidic motifs (B) HeLa cells were transfected with empty vector, SF or LF for 36h then treated with MnCl2 500 μM for 4h. Total cell lysates were prepared, subjected to SDS-PAGE and Western blot with the indicated antibodies. Lower panel shows the percentage of TMEM165 stability after normalization with actin (N = 2; * = P value < 0,05; *** = P value < 0,001).