Figure 7.

LHX6 inhibits the expression of β-catenin. (A, B) Ectopic expression of LHX6 significantly reduced the expression of β-catenin on the mRNA level. The mRNA expression was examined by RT-qPCR and RT-PCR with β-actin as an internal control. Error bars indicate s.d. (n=3). * P<0.05; ** P<0.01. (C) Overexpression of LHX6 significantly decreased the expression of β-catenin on the protein level; GAPDH was used as an internal control. (D) LHX6 decreased the endogenous protein expression of β-catenin in xenografted tumors, as detected by WB.