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. 2017 Aug 2;8(13):2561–2574. doi: 10.7150/jca.19972

Figure 8.

Figure 8

LHX6 transcriptionally represses the expression of β-catenin. (A) The schema of different regions of the β-catenin promoter (Catenin-promoter-full, -1 and -2) were constructed into a luciferase reporter vector. The region in the dotted line indicates the overlap region of Catenin-promoter-1 and -2. TSS indicates the transcription start site. (B) LHX6 inhibited the luciferase activity in LTEP-a-2 and SPC-a-1 cells transfected with Catenin-promoter-full. (C) LHX6 inhibited the luciferase activity only when the Catenin-promoter-2 region was co-transfected into LTEP-a-2 and SPC-a-1 cells. This result indicated that the region ranging from -1161bp to +27bp is crucial for the inhibitory activity of LHX6. Error bars indicate s.d. (n=3). ** P<0.01. (D) Schematic diagram of the mechanisms of LHX6-mediated suppression of lung adenocarcinoma cell proliferation and metastasis based on our study and previous studies. LHX6 transcriptionally silences the expression of β-catenin, which subsequently inhibits the expression of Wnt/β-catenin pathway genes; the pathway shown with the dotted lines indicates the possible mechanism reported in mice odontogenesis, and this mechanism needs further investigation in a cancer model.