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. 2016 Jul 1;66(10):1748–1760. doi: 10.1136/gutjnl-2015-310847

Figure 4.

Figure 4

Colitis initiation in α6ΔIEC mice is triggered by strong epithelial IL-18 secretion and colitis worsening by IL-1β hypersecretion. (A and B) Scattered dot plot quantification of IL-1β and IL-18 levels measured by ELISA starting from colon explants of WT and α6ΔIEC mice at 9 weeks (A) and at 2, 3 or 4 weeks of age (B) (error bars, SD; ns, not significant; *p<0.05, **p<0.01, ***p<0.001). (C) Western blot analysis performed on protein extracts obtained from an enriched epithelial fraction (enriched IECs) or from whole colon segments (total colon lysates) of 2-week-old WT and α6ΔIEC mice. Representative blots of three independent experiments performed on at least five animals per group are illustrated. Western blot with GAPDH and β-Tubulin are presented as loading controls. Casp1, caspase-1; enriched IECs, enriched intestinal epithelial cells; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; w, weeks.