Fig. 4. Chemical shift perturbations derived from 3,7-dioxo-octyl ACP 20 and 6-(3-hydroxyphenyl)-3-oxo-hexyl ACP 16. (A) Comparison of the 1H–15N HSQC spectra of holo (red) and 3,7-dioxo-octyl ACP 20 (blue) and (B) associated CSPs. Blue bars represent the CSPs of the major form observed whilst a green bar is also shown when two different conformers were observable (i.e. for R67 and V68). (C) Comparison of the 1H–15N HSQC spectra of holo (red) and 6-(3-hydroxyphenyl)-3-oxo-hexyl holo ACP 16 (purple) collected at 600 MHz and (D) associated CSPs.
