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. 2017 Aug 11;7(13):3387–3397. doi: 10.7150/thno.19748

Figure 5.

Figure 5

Immunocytochemistry of osteogenic protein, Runx2, Col Ⅰ and OPN, expressed by BMSCs cultured on different substrates in non-osteogenic growth media for 7 days (Runx2) and 14 days (Col Ⅰ and OPN). The results indicate that the MPZs promote the expression of Runx2, Col Ⅰ and OPN compared to the controls. The Runx2, Col Ⅰ and OPN were stained in green color with FITC. The nuclear stain is in dark blue with DAPI. KNN: unmodified but polarized KNN; MPZ: modified but polarized KNN; U-MPZ: modified but un-polarized KNN; HA: non-polarized hydroxyapatite.