Fig. 2.

SRSF2 promotes SMN intron7 retention. (A) (Upper panel) Schematic diagram of the E7–8 and E7–8 (20) minigenes. The primer binding sites are indicated with arrows. (Lower panel) RT-PCR analysis of the E7–8 and E7–8 (20) minigenes in SRSF2-expressing cells. (B) (Upper panel) Nucleotide sequence of a portion of the AdML pre-mRNA. The two exons are shown as dark yellow and yellow boxes, the upstream intron is indicated in orange, and the downstream intron is indicated in green. (Right panel) RT-PCR analysis of the AdML minigene using RNAs in SRSF2-expressing cells.