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A 10 nt RNA sequence physically interacts with SRSF2 and inhibits constitutive splicing. (A) (Upper panel) The 10 nt regulatory sequence with surrounding sequences and biotin-labeled RNA sequence (10B) are shown. (Lower panel) RNA-pulldown and immunoprecipitation analyses with SRSF2-specific antibody are shown. (B) (Upper panel) Schematic diagram of the AdML-M minigene. (Lower panel) RT-PCR analysis of intron splicing in the AdML-M minigene.
