Table 1.

Summary of diffraction data and structure refinement statistics Related to Figure 1–4

Beam line	APS-ID24E
Wavelength (Å)	0.9792
Space group	P22A
Cell parameters a, b, c (Å)
a, b, c (Å)	72.19, 101.24, 303.56
Resolution (Å)	50.0–2.60 (2.69–2.60)a
Rmerge(%)	9.2 (49.7)
Average I/σ(I)	14.3 (1.6)
Completeness (%)	94.6 (72.1)
CC(1/2)	0.805
Average redundancy	4.3 (3.4)
No. of unique reflections	65,587
Refinement and structure model
Rwork/Rfree(%)	17.8/22.6
No. of non-H atoms	13,175
Average B factor(Å2)
SpyCas9	60.1
AcrIIA4	53.6
RNA	60.6
RMS deviations
Bond lengths (Å)	1.081
Bond angles (°)	0.016
Ramachandran plot (%)
Favored	95.3
Allowed	4.7
Outliers	0

^aHighest resolution shell (in Å) shown in parentheses.