Extended Data Table 1. Data collection and refinement statistics (molecular replacement).
| ADIPOR2-scFv | ADIPOR2-scFv | |
|---|---|---|
| Data collection | ||
| No. of crystals (No. of wedges) | 5 (15) | 6 (12) |
| Space group | P21221 | P21221 |
| Cell dimensions | ||
| a, b, c (Å) | 74.58, 101.13, 111.58 | 74.16, 100.86, 110.39 |
| α, β, γ (°) | 90.00, 90.00, 90.00 | 90.00, 90.00, 90.00 |
| Resolution (Å) | 101.03-2.40 (2.50-2.40) * | 110.39-3.00 (3.18-3.00) |
| Anisotropy direction ** | ||
| Resolution where CC1/2 > 0.30 | ||
| overall (Å) | 2.41 | 3.01 |
| along h axis (Å) | 2.37 | 2.79 |
| along k axis (Å) | 2.36 | 2.74 |
| along l axis (Å) | 2.99 | 3.78 |
| Rmerge | 0.527 (NA) | 0.627 (NA) |
| Rpim | 0.174 (NA) | 0.248 (NA) |
| CC1/2 | 0.988 (0.489) | 0.979 (0.398) |
| I/σI | 5.5 (1.1) | 3.0 (1.0) |
| Completeness (%) | 100 (99.7) | 99.8 (99.3) |
| Redundancy | 10.5 (10.2) | 8.6 (8.2) |
| Refinement | ||
| Resolution (Å) | 32.21-2.40 | 74.46-3.00 |
| No. reflections | 33584 | 17135 |
| Rwork/Rfree | 17.60/20.25 | 20.38/24.80 |
| No. atoms | ||
| Protein | 4038 | 4087 |
| Zn | 1 | 1 |
| Oleate | 20 | 20 |
| Mono-oleins | 300 | 25 |
| Water | 488 | 227 |
| B-factors | ||
| Protein | 46.7 | 42.7 |
| Zn | 36.5 | 33.6 |
| Oleate | 48.3 | 35.3 |
| Mono-oleins | 81.5 | 53.9 |
| Water | 58.3 | 27.6 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.010 | 0.009 |
| Bond angles (°) | 1.060 | 1.000 |
*
Values in parentheses are for highest-resolution shell.
**
The anisotropy statistics were computed with AIMLESS.
NA-not applicable, Rmerge value over 1 is statistically meaningless.