Fig 3. Carbidopa protects mice from EAE.
A. Animal were given carbidopa in drinking water (filled squares) or plain water (open squares) and immunized with MOG35-55. Shown is the mean clinical score (n = 5). Error bars represent standard error of mean. Lymph node cells from mice immunized and treated as in A, were cultured in the presence or absence of MOG35-55 (20 μg/ml). Three days later, B, proliferation of T cells was quantified by thymidine incorporation and C, presence of IFN-γ, IL-17 and IL-6 was assessed by ELISA. Proliferation induced by no peptide was subtracted from that induced by MOG35-55 (20 μg/ml) to obtain ΔCPM. Error bars represent standard deviation of mean (n = 5). D, Number of live cells and E, frequency of CD4+ T cells in inguinal lymph nodes from mice treated as in A. F, FoxP3 expression by CD4+ T cells from E. G, summary of FoxP3 by CD4+ T cells from F. Error bars represent standard deviation of mean (n = 4). *P = <0.05, and **P = <0.005, P value <0.05 were considered significant.