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. 2017 Aug 14;7(5):603–609. doi: 10.1016/j.apsb.2017.07.001

Figure 4.

Fig. 4

SDS-PAGE analysis and GC—MS detection results of extract from an enzymatic reaction catalysed by purified pMAL-c2X-TwSMT1 protein and pMAL-c2X protein when using cycloartenol as the substrate. (A) The peak of extraction in the empty vector protein reaction system; (B) The peak of extraction in the recombinant pMAL-c2X-TwSMT1 protein reaction mixture; (C) A control using a 24-methylene cycloartenol standard; (D) 1: The recombinant pMAL-c2X-TwSMT1 overexpressed by isopropyl-1-thio-β-D-galactopyranoside (IPTG) 2: the empty pMAL-c2X overexpressed by IPTG; (E) The function of SMT1 from T. wilfordii; (F) Mass spectrogram of the 24-methylene cycloartenol standard; (G) Mass spectrogram of the product catalysed by recombinant TwSMT1 protein.