Fig 7. Crz1 depends on calcineurin for its nuclear localization and its regulation of filamentation on glucosamine medium.
(A) Strains cna1Δ, cna1ΔPGPD1-mCherry-Crz1, cnb1Δ, cnb1ΔPGPD1-mCherry-Crz1, cbp1Δ, and cbp1ΔPGPD1-mCherry-Crz1 were cultured on YP+GlcN medium for 7 days. (B) a diagram depicting the different localization patterns of Crz1: diffused in the cytosol with nuclear exclusion (left); localized to the nucleus (middle); localized to both cytoplasm and the nucleus but more concentrated in the nucleus (right), (C) Strains cna1ΔPGPD1-mCherry-Crz1, cnb1ΔPGPD1-mCherry-Crz1, and cbp1ΔPGPD1-mCherry-Crz1 were cultured either in YPD medium or in YP+GlcN medium overnight. The mCherry-Crz1 signal showed diffused cytoplasmic localization in the cna1Δ and cnb1Δ mutants under both conditions. The mCherry-Crz1 signal showed diffused cytoplasmic localization but with more concentrated signals in the nucleus in the cbp1Δ mutant.