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. 2017 Sep 12;12(9):e0184155. doi: 10.1371/journal.pone.0184155

Fig 3. 2D-DIGE analysis of osteogenic differentiation of HWJSC spheroids at day 7 versus day 1.

Fig 3

HWJSC spheroids were cultured for either 1 or 7 days, and 2D-differential gel electrophoresis and mass spectrometry was performed to identify the differentially expressed proteins that were present on at least one gel (out of a total of 3 gels, representing randomized samples from 3 independent experiments) and identified with at least 2 peptides. Fold change was calculated for each unique spot, with an ANOVA p-value cutoff of 0.055. The protein name, symbol, SwissProt Accession, SameSpots #, and ANOVA p-value are provided in columns for each unique identified protein, and protein expression fold change in day 7 versus day 1 samples is presented as a bar graph centered around a fold change of 1 (representing no difference between day 1 and day 7).